全文获取类型
收费全文 | 9446篇 |
免费 | 748篇 |
国内免费 | 763篇 |
出版年
2024年 | 3篇 |
2023年 | 119篇 |
2022年 | 186篇 |
2021年 | 561篇 |
2020年 | 355篇 |
2019年 | 469篇 |
2018年 | 462篇 |
2017年 | 316篇 |
2016年 | 447篇 |
2015年 | 683篇 |
2014年 | 792篇 |
2013年 | 781篇 |
2012年 | 923篇 |
2011年 | 850篇 |
2010年 | 496篇 |
2009年 | 448篇 |
2008年 | 498篇 |
2007年 | 415篇 |
2006年 | 326篇 |
2005年 | 265篇 |
2004年 | 233篇 |
2003年 | 241篇 |
2002年 | 197篇 |
2001年 | 147篇 |
2000年 | 113篇 |
1999年 | 126篇 |
1998年 | 71篇 |
1997年 | 67篇 |
1996年 | 62篇 |
1995年 | 50篇 |
1994年 | 36篇 |
1993年 | 25篇 |
1992年 | 41篇 |
1991年 | 22篇 |
1990年 | 21篇 |
1989年 | 38篇 |
1988年 | 14篇 |
1987年 | 9篇 |
1986年 | 9篇 |
1985年 | 22篇 |
1984年 | 4篇 |
1983年 | 7篇 |
1982年 | 3篇 |
1981年 | 2篇 |
1975年 | 1篇 |
1945年 | 1篇 |
排序方式: 共有10000条查询结果,搜索用时 31 毫秒
11.
Elinor McKone Sacha Stokes Jia Liu Sarah Cohan Chiara Fiorentini Madeleine Pidcock Galit Yovel Mary Broughton Michel Pelleg 《PloS one》2012,7(10)
Other-race and other-ethnicity effects on face memory have remained a topic of consistent research interest over several decades, across fields including face perception, social psychology, and forensic psychology (eyewitness testimony). Here we demonstrate that the Cambridge Face Memory Test format provides a robust method for measuring these effects. Testing the Cambridge Face Memory Test original version (CFMT-original; European-ancestry faces from Boston USA) and a new Cambridge Face Memory Test Chinese (CFMT-Chinese), with European and Asian observers, we report a race-of-face by race-of-observer interaction that was highly significant despite modest sample size and despite observers who had quite high exposure to the other race. We attribute this to high statistical power arising from the very high internal reliability of the tasks. This power also allows us to demonstrate a much smaller within-race other ethnicity effect, based on differences in European physiognomy between Boston faces/observers and Australian faces/observers (using the CFMT-Australian). 相似文献
12.
Liyi Xu Jiamin Chen Litao Jia Xiao Chen Faycal Awaleh Moumin Jianting Cai 《Journal of cellular and molecular medicine》2020,24(24):14392
Gastric cancer is a major cause of mortality worldwide. The glutamate/aspartate transporter SLC1A3 has been implicated in tumour metabolism and progression, but the roles of SLC1A3 in gastric cancer remain unclear. We used bioinformatics approaches to analyse the expression of SLC1A3 and its role in gastric cancer. The expression levels of SLC1A3 were examined using RT‐qPCR and Western bolting. SLC1A3 overexpressing and knock‐down cell lines were constructed, and the cell viability was evaluated. Glucose consumption, lactate excretion and ATP levels were determined. The roles of SLC1A3 in tumour growth were evaluated using a xenograft tumour growth model. SLC1A3 was found to be overexpressed in gastric cancer, and this overexpression was associated with poor prognosis. In vitro and in vivo assays showed that SLC1A3 affected glucose metabolism and promoted gastric cancer growth. GSEA analysis suggested that SLC1A3 was positively associated with the up‐regulation of the PI3K/AKT pathway. SLC1A3 overexpression activated the PI3K/AKT pathway and up‐regulated GLUT1, HK II and LDHA expression. The PI3K/AKT inhibitor LY294002 prevented SLC1A3‐induced glucose metabolism and cell proliferation. Our findings indicate that SLC1A3 promotes gastric cancer progression via the PI3K/AKT signalling pathway. SLC1A3 is therefore a potential therapeutic target in gastric cancer. 相似文献
13.
Ong Jia Sin Taylor Todd D. Yong Cheng Chung Khoo Boon Yin Sasidharan Sreenivasan Choi Sy Bing Ohno Hiroshi Liong Min Tze 《Probiotics and antimicrobial proteins》2020,12(1):125-137
Probiotics and Antimicrobial Proteins - This study aimed to elucidate the targets and mechanisms of anti-staphylococcal effects from bioactive metabolites produced by lactic acid bacteria. We aimed... 相似文献
14.
15.
16.
17.
18.
Fei Han Li Yin Xiao Jiang Xi Zhang Ning Zhang Juntang Yang Weiming Ouyang Xianglin Hao Wenbin Liu Yongsheng Huang Hongqiang Chen Fei Gao Zhongtai Li Qiaonan Guo Jia Cao Jinyi Liu 《Aging cell》2021,20(5)
Although important factors governing the meiosis have been reported in the embryonic ovary, meiosis in postnatal testis remains poorly understood. Herein, we first report that SRY‐box 30 (Sox30) is an age‐related and essential regulator of meiosis in the postnatal testis. Sox30‐null mice exhibited uniquely impaired testis, presenting the abnormal arrest of germ‐cell differentiation and irregular Leydig cell proliferation. In aged Sox30‐null mice, the observed testicular impairments were more severe. Furthermore, the germ‐cell arrest occurred at the stage of meiotic zygotene spermatocytes, which is strongly associated with critical regulators of meiosis (such as Cyp26b1, Stra8 and Rec8) and sex differentiation (such as Rspo1, Foxl2, Sox9, Wnt4 and Ctnnb1). Mechanistically, Sox30 can activate Stra8 and Rec8, and inhibit Cyp26b1 and Ctnnb1 by direct binding to their promoters. A different Sox30 domain required for regulating the activity of these gene promoters, providing a “fail‐safe” mechanism for Sox30 to facilitate germ‐cell differentiation. Indeed, retinoic acid levels were reduced owing to increased degradation following the elevation of Cyp26b1 in Sox30‐null testes. Re‐expression of Sox30 in Sox30‐null mice successfully restored germ‐cell meiosis, differentiation and Leydig cell proliferation. Moreover, the restoration of actual fertility appeared to improve over time. Consistently, Rec8 and Stra8 were reactivated, and Cyp26b1 and Ctnnb1 were reinhibited in the restored testes. In summary, Sox30 is necessary, sufficient and age‐associated for germ‐cell meiosis and differentiation in testes by direct regulating critical regulators. This study advances our understanding of the regulation of germ‐cell meiosis and differentiation in the postnatal testis. 相似文献
19.
Shi-Ming Yang Wei Chen Wei-Wei Guo Shuping Jia Jian-He Sun Hui-Zhan Liu Wie-Yen Young David Z. Z. He 《PloS one》2012,7(9)
The hallmark of mechanosensory hair cells is the stereocilia, where mechanical stimuli are converted into electrical signals. These delicate stereocilia are susceptible to acoustic trauma and ototoxic drugs. While hair cells in lower vertebrates and the mammalian vestibular system can spontaneously regenerate lost stereocilia, mammalian cochlear hair cells no longer retain this capability. We explored the possibility of regenerating stereocilia in the noise-deafened guinea pig cochlea by cochlear inoculation of a viral vector carrying Atoh1, a gene critical for hair cell differentiation. Exposure to simulated gunfire resulted in a 60–70 dB hearing loss and extensive damage and loss of stereocilia bundles of both inner and outer hair cells along the entire cochlear length. However, most injured hair cells remained in the organ of Corti for up to 10 days after the trauma. A viral vector carrying an EGFP-labeled Atoh1 gene was inoculated into the cochlea through the round window on the seventh day after noise exposure. Auditory brainstem response measured one month after inoculation showed that hearing thresholds were substantially improved. Scanning electron microscopy revealed that the damaged/lost stereocilia bundles were repaired or regenerated after Atoh1 treatment, suggesting that Atoh1 was able to induce repair/regeneration of the damaged or lost stereocilia. Therefore, our studies revealed a new role of Atoh1 as a gene critical for promoting repair/regeneration of stereocilia and maintaining injured hair cells in the adult mammal cochlea. Atoh1-based gene therapy, therefore, has the potential to treat noise-induced hearing loss if the treatment is carried out before hair cells die. 相似文献
20.
Cyclin E is a regulator of cyclin-dependent protein kinases (Cdks) and is involved in mediating the cell cycle transition from G1 to S phase. Here, we describe a novel function for cyclin E in the long term maintenance of checkpoint arrest in response to replication barriers. Exposure of cells to mitomycin C or UV irradiation, but not ionizing radiation, induces stabilization of cyclin E. Stabilization of cyclin E reduces the activity of Cdk2-cyclin A, resulting in a slowing of S phase progression and arrest. In addition, cyclin E is shown to be required for stabilization of Cdc6, which is required for activation of Chk1 and the replication checkpoint pathway. Furthermore, the stabilization of cyclin E in response to replication fork barriers depends on ATR, but not Nbs1 or Chk1. These results indicate that in addition to its well studied role in promoting cell cycle progression, cyclin E also has a role in regulating cell cycle arrest in response to DNA damage. 相似文献